Figure 1: The pedestal and mirror are being cleaned with a lint-free tissue
Figure 2: Bad Blank warning.
To obtain optimal readings we suggest the following steps:
RIPA
NDSB
Message Text | Explanation/Solution |
Air bubble, lint residue or bad sample. Please reapply sample. | Air bubble recognition is on and has detected either an air bubble, lint residue or a bad sample like e.g. a turbid sample. Check sample, clean the sample window on pedestal and mirror in the lid arm thoroughly and reapply sample carefully. To avoid air bubbles apply sample by reverse pipetting. |
High absorbance at 250 – 280 nm, 280 – 340 nm, 340 – 400 nm, 400 – 475 nm, 475 – 550 nm, 550 – 625 nm, 625 – 700 nm. Bad Blank or insufficient cleaning. | The warning message is shown when a blank measurement (NanoVolume) detects a significant absorbance a potential area of interest. The warning message shows the wavelength range where the absorbance is appearing. Two things can cause high absorbance in a blank measurement: either the blank solution/buffer has an absorbance in this wavelength range or the sample window on pedestal and the mirror in the lid arm was not cleaned properly after the last reading. Clean the sample window on pedestal and the mirror in the lid arm thoroughly and check the blank solution for absorbance (Use water for blank and measure the blank buffer as sample). |
Lint residue or bad sample. Please reapply sample. | Displayed when Air Bubble Recognition is off. Software has detected either a lint residue or a bad sample like e.g. a turbid sample. Check sample, clean the sample window on pedestal and mirror in the lid arm thoroughly and reapply sample carefully. |
Maximum absorbance level at specified wavelength reached. Calculations may lead to low/wrong results. | The concentration of the sample used is too high and exceeds the specified absorbance range. Maximum absorbance is 330 A (10 mm path) for NanoVolume and 2.65 A for cuvette measurements. Dilute sample and measure again. |
Maximum level exceeded. | This message is shown when for example, too many dyes or wavelength are added with the Add + button. It is possible to add up to 20 dyes or wavelengths. |
Sample concentration too low for 0.3 µl – change volume and parameter settings to 1 µl | The measurement parameters for 0.3 µl NanoVolume samples read only the 0.07 mm path length (dilution 140). The utilized sample concentration is too low. Please use 1 µl of the sample and change the volume setting to 1 – 2 µl. Minimum concentrations for the 0.07 mm path length for dsDNA 420 ng/µl and for BSA 12.6 mg/ml |