Implen Journal Club

Implen Journal Club

Welcome to the Implen NanoPhotometer® Journal Club. Here we will highlight relevant publications where the Implen NanoPhotometer® helped researchers to unravel the mysteries of modern molecular biology.

Current Month Journal Club Issue

May 2022 | Spring Edition | Full Newsletter (html) (pdf)

Implen nanophotometer journal club spring issue

The first issue is highlighting the work of Abraham et al. who described for the first time in the Journal of Pharmaceuticals the production and characterization of cucumber-derived extracellular vesicles (EVs) and their ability to improve dermal drug delivery. Extracellular vesicles (EVs) are considered to be efficient nanocarriers for improved drug delivery and can be derived from mammalian or plant cells. Cucumber-derived plant exosome-like vesicles (PEVs) were successfully obtained and characterized, wherein the cucumber-derived PEVs were demonstrated to increase the dermal penetration efficacy of a lipophilic active ingredient (AI) surrogate to about 200%.

The NanoPhotometer NP80 was used in this study to obtain absorbance values to quantify the protein amounts of the PEVs using the BCA assay with micro BCA kits.

The second issue is exploring the concept of novel food, with insects reared under controlled conditions being considered mini livestock. Mass-reared edible insect production is an economically and ecologically beneficial alternative to conventional meat gain. House crickets (Acheta domesticus) and Jamaican field crickets (Gryllus assimilis) are preferred insect species that are used commercially as food. The novel information from this study on the edible insect-associated microbiota will contribute to developing strategies for cricket processing to avoid bacteria-caused risks of microbial communities of having detrimental properties and reap the benefits of beneficial microbes. Edible insect-associated microorganisms should gain much attention considering their potential beneficial, health-promoting features.

The NanoPhotometer P330 was used to assess the quality and quantity of microbial DNA isolated from crickets.

Implen nanophotometer journal club spring issue
Implen nanophotometer journal club spring issue

The next issue is revisiting the topic about small, but very important insects whose health should concern us all – honey bees. As many species of social insects display a division of labor among colony members based on behavioral specializations related to age, adult worker honey bees perform a series of tasks in the hive when they are young and with age shift to foraging for nectar and pollen outside the hive. Ortiz-Alvarado et al. demonstrated in the Journal of Insect Science that antibiotic treatments, which are often used to treat or prevent infections and to improve honey production, had an effect on the typical expression pattern of regulatory genes and that the immature environment can influence adult development.  Antibiotic effects on individual behavior highlight the need for further focus on the gut–brain axis and microbiota role on honey bee social organization.

The NanoPhotometer® was used to quantify RNA extracted from the honey bee tissues.

Next issue explores the topic of the convergent evolution of vertebrate-pollinated flowers. Nearly 90% of flowering plants depend on animals for reproduction. One of the main rewards plants offer to pollinators for visitation is nectar, with one rare trait among flowering plants is the production of colored nectar, which may function as a visual attractant and guide prospective pollinators. Roya et al. reported in the journal of plant biology findings indicating convergent evolution of a red-colored nectar has occurred across two distantly related plant species. Behavioral data show that the red pigment attracts the likely pollinator of one of these plants.

The NanoPhotometer® Pearl was used for all spectrophotometric analysis for this study including: (1) spectrophotometric catalays assay performed by monitoring the breakdown of H2O2 by measuring the absorbance at 250 nm and (2) absorbance wavelength scans performed 8-20 hours following in-situ pharmacological treatments of nectar with carbonic anhydrase inhibitors (acetazolamide, sulfanilamide, or 6-ethoxy-2-benzothiazolesulfonamide) or equivalent amounts of DMSO.

Implen nanophotometer journal club spring issue
Implen nanophotometer journal club spring issue

The last issue is highlighting the topic of the impact of Electromagnetic fields (EMFs) which are ubiquitous in the environment and interfere with all biological organisms including plantlife. The quality and quantity of alternating EMFs are increasing due to the implementation of novel technologies precipitating the need for researching their effects. The results of this study, in which the model plant Arabidopsis thaliana was exposed to an applied EMF program aimed to mimic a mix of natural and anthropogenic components, show that alternating EMF-exposed plants undergo significant alterations at the levels of photosynthesis, transcriptome, and metabolome. These findings of EMF–plant interaction along with the rapidly changing EMF emissions accentuate the requirement to research the EMF–life and, as conducted here, EMF–plant interaction.

The NanoPhotometer® was used in this study to verify the yield and purity of RNA isolated from the model plants exposed to EMFs for transcriptome analysis by RNA sequencing and quantitative real-time PCR validation.

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