Implen Journal Club

Implen Journal Club

Welcome to the Implen NanoPhotometer® Journal Club. Here we will highlight relevant publications where the Implen NanoPhotometer® helped researchers to unravel the mysteries of modern molecular biology.

1-The-Implen-NanoPhotometer-was-used-to-assess-RNA-concentration-and-purity-in-research-of-potential-consequences-of-eating-at-night-552

We are covering the topic of the potential consequences of eating at night. Davis et. al recently reported in the journal of Clinical Nutrition that eating at night has been linked to impaired glucose metabolism and dyslipidaemia that is likely a consequence of an underlying disrupted circadian rhythm in metabolic processes. It was shown that the time of day a meal is consumed had an effect on which genes were differentially regulated immediately following the meal, with only 6.5% of differentially expressed genes the same both morning and night, with more genes involved in lipid metabolic pathways in the morning and immune pathways at night suggesting that key regulatory genes responsible for nutrient sensing and lipid and glucose metabolism are differentially expressed at night. The Implen NanoPhotometer® was used to assess RNA concentration and purity.

Next, we are highlighting the work of Ruknarong et al. who reported in the journal of Biochemistry, Biophysics and Molecular Biology that Vitamin C supplementation reduces expression of circulating miR-451a in subjects with poorly controlled type 2 diabetes mellitus (T2DM) and high oxidative stress. This study investigated the effect of vitamin C, an essential element required for normal metabolic function, supplementation on circulating miRNA expression in subjects with T2DM and with poor glycemic control and provides evidence that daily supplementation with 1000 mg/day of vitamin C for six weeks can improve blood glucose, lipid profiles, glycated haemoglobin (HbA1C) and insulin in subjects with T2DM. In addition, this study identified a candidate miRNA that may be used as a biomarker to identify subjects that respond to vitamin C treatment or oxidative status in plasma. The Implen NanoPhotometer® N60 was used in this study to measure miRNA concentrations.

2-The-Implen-NanoPhotometer-N60-was-used-in-this-study-to-measure-miRNA-concentrations-in-research-of-vitamin-c-to-reduce-expression-of-circulating-miR-451a-552
3-The-Implen-NanoPhotometer-was-used-in-research-of-treatment-with-Telmisartan-of-alcohol-damaged-liver-552
Next, we are continuing with the topic of severe liver damage due to excessive consumption of alcohol and potential therapeutic approaches. Excessive consumption of alcohol may induce severe liver damage, in part via oxidative stress and inflammatory responses, which implicates these processes as potential therapeutic approaches. Amir Mohamed Abdelhamid et. al. recently published a study in the journal of International Immunopharmacology aimed to determine TEL’s hepatoprotective effects and to identify its possible curative mechanisms in alcoholic liver disease. They showed that the mechanism of action of Telmisartan may be to modulate ethanol-induced hepatic injury by suppressing NF-κB and may attenuate ethanol-induced hepatic injury via activation of PPAR-γ/Nrf-2 and Hmox-1.
In the final issue, we are exploring the topic of Dysfunctional adipose tissue (AT), which may contribute to the pathology of several metabolic diseases through altered lipid metabolism, insulin resistance, and inflammation. AT is a highly active metabolic organ exerting numerous vital functions in the body including: lipid storage function, glucose homeostasis, hormone secretion, energy homeostasis and thermogenesis. Gencer et. al. recently published findings in the journal of Biomedicines which highlight a potential therapeutic candidate in the research of metabolic diseases as the atypical chemokine receptor 3 (ACKR3) and that the adipocyte-specific ACKR3 is indeed involved in the regulation of AT lipid levels – suggesting that ACKR3 may be a possible contributor to metabolic diseases, such as insulin resistance, obesity, and atherosclerosis. The Implen NanoPhotometer® N60/N50 used to determine quality (A260/A280) and the quantity (ng/µL) of the RNA were measured by. A ratio of ~2 for A260/A280 was accepted as good quality RNA.
4-The-Implen-NanoPhotometer-N60-N50-used-to-determine-quality-(A260-A280)-and-the-quantity-(ng-µL)-of-the-RNA-in-research-of-dysfunctional-adipose-tissue-552
5 The NanoPhotometer P330 was used to measure the RNA yields of the total mRNA extracted in research of brain cancer 552
Glioblastoma (GBM) – one of the most aggressive and invasive forms of brain cancer, which is highly resistant to anticancer drugs. Soohyun Hong et al. recently published findings that may provide new insights into a better understanding of GBM-immunity interaction and for the development of a novel therapeutic strategy for GBM. GBM constitutes a highly complicated and dynamic in vivo tumor-microenvironment (TME) that modulates and participates in GBM proliferation, invasion, and resistance to drugs. GBM progression is closely associated with microglia activation; therefore, understanding the regulation of the crosstalk between human GBM and microglia may help develop effective therapeutic strategies. It was shown that miR-124 microRNA is enriched in the normal brain, but its levels are downregulated in GBM- so the strategy in this study was to deliver miR-124 to the GBM microenvironment via extracellular vecles (EVs) as an efficient miRNA delivery vehicle as EV drugs have the unique advantage of superior potential of blood brain barrier (BBB) penetration. The NanoPhotometer® P330 was used to measure the RNA yields of the total mRNA extracted.

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