Implen Journal Club

Implen Journal Club

Welcome to the Implen NanoPhotometer® Journal Club. Here we will highlight relevant publications where the Implen NanoPhotometer® helped researchers to unravel the mysteries of modern molecular biology.

Current Month Journal Club Issue

June 2021 | Pathogens Issue | Full Newsletter (html) (pdf)

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Anna-Lena Altenhoff, Sven Thierbach and Alexander Steinbüchel from Westfälische Wilhelms-Universität Münster have recently published their study about enzymatic degradation of rubber with the latex clearing protein Lcp1VH2 as an environmentally friendly and economical solution to treat the enormous amount of rubber waste. The spectrum of the purified Lcp1VH2 was measured with the NanoPhotometer® to quantify the yield of the protein, as well as to verify the differences in the heme content of the protein… 

The global spread of SARS-CoV-2, an infection ranging from asymptomatic to severe with lingering symptomatology in some, calls for a thorough molecular understanding of the pathophysiology of the disease.  This prompted the study recently published in Scientific Reports which was specifically designed by Hye Kyung Lee et al. to fill a gap in the understanding of immunological outcomes in asymptomatic individuals following a community super-spreading event… The NanoPhotometer® was used to analyze each sample in this study for RNA concentration.

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Pathogens issue of the Implen NanoPhotometer® Journal Club continues with the topic of antimicrobial resistance (AMR), an increasingly global health concern estimated to cause 10 million deaths annually by 2050. In recent years, studies have demonstrated that airborne ARG-carrying human pathogenic bacteria are closely associated with multiple respiratory diseases and infections which can lead to antimicrobial resistance and potential health risk. The NanoPhotometer® N60 was utilized in this study to check the DNA concentration and quality…

In an effort to rapidly develop and/or repurpose safe and effective drugs to treat SARS-CoV-2, Alistair S. Brown, David F. Ackerley and Mark J. Calcott at the School of Biological Sciences in New Zealand developed a high-throughput screen using a protein-based FRET-biosensor to identify inhibitors of SARS-CoV-2 that is cost-effective and robust with high reproducibility. Their work, which was recently published in Molecules, demonstrated the utility of this biosensor for inhibitor discovery by screening 1280 compounds from the Library of Pharmaceutically Active Compounds collection.  In this study, the protein concentrations were determined by measuring the absorbance at 280 nm using the NanoPhotometer® NP80…

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The recently reported new variants of SARS-CoV-2 carrying several mutations in the spike protein were documented in the UK and South Africa. Early epidemiological and clinical findings have indicated that these variants show increased transmissibility in the population and the UK variant of SARS-CoV-2 is thought to be more infectious than previously circulating strains as a result of several mutations. This was confirmed via biophysical assays and by determining cryo-EM structures of spike protein ectodomains bound to 2 representative potent neutralizing antibody fragments. Protein concentration was measured spectrophotometrically with the NanoPhotometer® N60.

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