Implen Journal Club
Implen Journal Club
Welcome to the Implen NanoPhotometer® Journal Club. Here we will highlight relevant publications where the Implen NanoPhotometer® helped researchers to unravel the mysteries of modern molecular biology.
Current Month Journal Club Issue
May 2023 | Spring Edition | Full Newsletter (html) (pdf)
The first issue is highlighting the impact of pesticides on soil fungal and bacterial communities, as well as wheat diseases. Vasilchenko et. al. evaluated the potential adverse effects of a mixture of fungicides (difenoconazole, epoxiconazole, and kresoxim-methyl) on soil fungal and bacterial communities, as well as the manifestation of wheat diseases. The results showed that the fungicide mixture decreased the indices of both fungal and bacterial communities, while the relative abundances of certain bacterial taxa increased. This study also revealed a high potential for soil bacteria to degrade pollutants and for the soil fungal community to recover after the application of fungicides.
The NanoPhotometer® N120 was used in this study to assess the yield and quality of total soil DNA using the following absorbance ratios: A260 nm/230 nm (DNA/humic acid) and A260 nm/280 nm (DNA/protein).
The second issue is highlighting a study that demonstrated Phytoremediation is an innovative and sustainable approach to remediate soil contaminated with petroleum hydrocarbons with the use of Enterobacter ludwigii ZCR5 strain. The results from this study published by Pacwa-Płociniczak et. al. in the journal of Applied Soil Ecology suggest that the strain could be a promising solution for the remediation of petroleum-contaminated soil. It is a cost-effective and eco-friendly approach that has gained popularity in recent years.
The quantity and quality of the RNA were assessed using the NanoPhotometer®.
In the next issue, we are thrilled to share an exciting breakthrough in soil remediation research featured in a recent study published in Applied Soil Ecology that delves into the world of phytoremediation with a fascinating twist. With this study, Hofmann et. al. demonstrated the potential of the Enterobacter ludwigii ZCR5 strain in cleaning up soil contaminated with petroleum hydrocarbons (PHs).
This study paves the way for bacterial-assisted phytoremediation, a promising technique for restoring polluted soil to its natural state. By harnessing the power of microorganisms, we can mitigate the environmental impact of PH contamination while promoting plant growth and ecosystem health.
The NanoPhotometer® Pearl was used in this study to check the quantity of DNA extracted from the soil.
The final issue is introducing exciting new research just published by Ahmed El Moukhtari and team “Silicon improves seed germination and seedling growth and alleviates salt stress in Medicago sativa L.” In this study, researchers investigated the effects of silicon on the growth and stress tolerance of alfalfa plants. The impressive results showed that silicon application led to enhanced seed germination and seedling growth, even under salt stress conditions. This is great news for improving crop productivity in challenging environments.
The NanoPhotometer® NP80 was used in this study to measure the OD at 520 nm to assess proline content of the soil.
One of the top New Year’s Resolutions in 2023 is to reduce stress on the job. This issue is focusing on the potential effects of reducing acute and chronic stress on the supporting healthy immune system function. Stress is a growing condition of significant concern to public health as stressing episodes induce metabolic changes and may be linked to several illnesses including those that impact the central nervous system and cardiovascular diseases, such as depression and anxiety. Kirsten et. al. described in the International Journal on the Biology of Stress how stress, both acute and chronic, affected the brain’s expression of specific immune-related genes and neuronal function markers.
The NanoPhotometer® was used in this study to measure the RNA quality and concentration by spectrophotometry.
Previous Journal Club Issues
2022
2021
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