Some UV/Vis spectrophotometer software packages are offering chemometric algorithms to not only identify contaminants in samples, but to also give an estimate of how much the readings are influenced by the contaminant to suggest corrected values based on these calculations. This sometimes includes a parallel determination of DNA and RNA to potentially overcome the need to run other analytical methods for the analysis. All chemometric approaches are heavily depending on the nature of the sample (mammalian, bacterial, plant, yeast, tumor, FFPE, etc.) and the matrix that the sample is present in (different salts, organic components, pH values, etc.). The usability of those calculations is therefore limited to very dedicated combinations of samples and certain clearly defined sample environments. In addition those results cannot be verified easily, which makes it very difficult for a researcher to confirm and trust the corrected sample concentration and quality. These restrictions include nucleic acid and protein samples equally.
NanoPhotometer® Sample Control™ in comparison sends alerts to inform clients about a contamination or handling error, flags the sample, and notifies the researcher of where the contamination/issue might come from and how to improve the quality of the sample. Sample Control™ provides researchers with reliable feedback that can be verified and is independent of the nature of the sample.