There are easy and quick options available to check the quality of the blank solution and of the blank. Protein buffers like RIPA, for example, absorb at 280 nm (Figure 1).
Figure 1: Absorbance spectra of different protein buffers (Blank = dH2O)
Figure 2: Blank warning message because of high absorbance in the range of 250 – 280 nm
Implen’s unique Blank Control™ feature of the NanoPhotometer, top UV-Vis spectrophotometer, will protect you from wasting time and precious sample on inaccurate readings caused by high background blanks or inappropriate cleaning.